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1 article(s) from Merritt, Kristen K

Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance

Kristen K. Merritt,
Kevin M. Bradley,
Daniel Hutter,
Mariko F. Matsuura,
Diane J. Rowold and
Steven A. Benner

Beilstein J. Org. Chem. 2014, 10, 2348–2360, doi:10.3762/bjoc.10.245

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Graphical Abstract

Figure 1: Structures explaining why large DNA (L-DNA) constructs cannot easily autonomously self-assemble fro...

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Figure 2: Watson–Crick pairing rules follow two rules of complementarity: (a) size complementarity (large pur...

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Figure 3: (top) Schematic showing the mix-anneal-extend-ligate-amplify process exploiting AEGIS pairing (to o...

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Figure 4: The exact sequences and overlaps of the fragments designed by OligArch to allow the autonomous asse...

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Figure 5: Agarose gel (1%, stained with ethidium bromide) showing the products (before PCR) arising from the ...

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Figure 6: LB-agar plates spread, at various dilutions, with 25 µL cells transformed by plasmids containing th...

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Figure 7: (left) Autonomous assembly of the 32A construct yielded the desired 1,121 base-pair amplicon in a s...

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Figure 8: Fragments built only from standard nucleotides, without AEGIS, and designed by OligArch [21] to support...

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Published 09 Oct 2014

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